4C7O

The structural basis of FtsY recruitment and GTPase activation by SRP RNA

4C7O の概要

• エントリーDOI: 10.2210/pdb4c7o/pdb
• 分子名称: SIGNAL RECOGNITION PARTICLE PROTEIN, SIGNAL RECOGNITION PARTICLE RECEPTOR FTSY, SRP RNA, ... (7 entities in total)
• 機能のキーワード: nuclear protein-rna complex, nuclear protein, protein translocation, signal recognition particle, signal recognition particle receptor, gdp alf3/4, nuclear protein/rna
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Cytoplasm: P0AGD7; Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P10121
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 143564.60

構造登録者

Voigts-Hoffmann, F.,Schmitz, N.,Shen, K.,Shan, S.O.,Ataide, S.F.,Ban, N. (登録日: 2013-09-23, 公開日: 2013-11-20, 最終更新日: 2023-12-20)

主引用文献

Voigts-Hoffmann, F.,Schmitz, N.,Shen, K.,Shan, S.O.,Ataide, S.F.,Ban, N.
The Structural Basis of Ftsy Recruitment and Gtpase Activation by Srp RNA
Mol.Cell, 52:643-, 2013

PubMed Abstract: The universally conserved signal recognition particle (SRP) system mediates the targeting of membrane proteins to the translocon in a multistep process controlled by GTP hydrolysis. Here we present the 2.6 Å crystal structure of the GTPase domains of the E. coli SRP protein (Ffh) and its receptor (FtsY) in complex with the tetraloop and the distal region of SRP-RNA, trapped in the activated state in presence of GDP:AlF4. The structure reveals the atomic details of FtsY recruitment and, together with biochemical experiments, pinpoints G83 as the key RNA residue that stimulates GTP hydrolysis. Insertion of G83 into the FtsY active site orients a single glutamate residue provided by Ffh (E277), triggering GTP hydrolysis and complex disassembly at the end of the targeting cycle. The complete conservation of the key residues of the SRP-RNA and the SRP protein implies that the suggested chemical mechanism of GTPase activation is applicable across all kingdoms.

PubMed: 24211265
DOI: 10.1016/J.MOLCEL.2013.10.005

実験手法

X-RAY DIFFRACTION (2.6 Å)