4C6H

Haloalkane dehalogenase with 1-hexanol

Summary for 4C6H

• Entry DOI: 10.2210/pdb4c6h/pdb
• Descriptor: HALOALKANE DEHALOGENASE, CHLORIDE ION, TRIETHYLENE GLYCOL, ... (5 entities in total)
• Functional Keywords: hydrolase
• Biological source: RHODOBACTERACEAE
• Total number of polymer chains: 1
• Total formula weight: 32946.53

Authors

Novak, H.R.,Sayer, C.,Isupov, M.,Gotz, D.,Spragg, A.M.,Littlechild, J.A. (deposition date: 2013-09-18, release date: 2014-05-14, Last modification date: 2023-12-20)

Primary citation

Novak, H.R.,Sayer, C.,Isupov, M.N.,Gotz, D.,Spragg, A.M.,Littlechild, J.A.
Biochemical and Structural Characterisation of a Haloalkane Dehalogenase from a Marine Rhodobacteraceae.
FEBS Lett., 588:1616-, 2014

PubMed Abstract: A putative haloalkane dehalogenase has been identified in a marine Rhodobacteraceae and subsequently cloned and over-expressed in Escherichia coli. The enzyme has highest activity towards the substrates 1,6-dichlorohexane, 1-bromooctane, 1,3-dibromopropane and 1-bromohexane. The crystal structures of the enzyme in the native and product bound forms reveal a large hydrophobic active site cavity. A deeper substrate binding pocket defines the enzyme preference towards substrates with longer carbon chains. Arg136 at the bottom of the substrate pocket is positioned to bind the distal halogen group of extended di-halogenated substrates.

PubMed: 24613925
DOI: 10.1016/J.FEBSLET.2014.02.056

Experimental method

X-RAY DIFFRACTION (1.61 Å)