4C4D

Covalent glycosyl-enzyme intermediate of Hypocrea jecorina Cel7a E217Q mutant trapped using DNP-2-deoxy-2-fluoro-cellotrioside

Summary for 4C4D

• Entry DOI: 10.2210/pdb4c4d/pdb
• Related: 4C4C
• Related PRD ID: PRD_900005
• Descriptor: CELLULOSE 1,4-BETA-CELLOBIOSIDASE, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: hydrolase, glycoside hydrolase, cellobiohydrolase, cellulase.
• Biological source: TRICHODERMA REESEI
• Total number of polymer chains: 1
• Total formula weight: 48128.24

Authors

Haddad-Momeni, M.,Mackenzie, L.,Sandgren, M.,Withers, S.G.,Stahlberg, J. (deposition date: 2013-09-05, release date: 2014-01-08, Last modification date: 2023-12-20)

• Primary citation: Knott, B.C.,Haddad Momeni, M.,Crowley, M.F.,Mackenzie, L.F.,Gotz, A.W.,Sandgren, M.,Withers, S.G.,Stahlberg, J.,Beckham, G.T.; The Mechanism of Cellulose Hydrolysis by a Two-Step, Retaining Cellobiohydrolase Elucidated by Structural and Transition Path Sampling Studies.; J.Am.Chem.Soc., 136:321-, 2014; PubMed: 24341799; DOI: 10.1021/JA410291U

Experimental method

X-RAY DIFFRACTION (1.32 Å)