4C2M

Structure of RNA polymerase I at 2.8 A resolution

4C2M の概要

• エントリーDOI: 10.2210/pdb4c2m/pdb
• 分子名称: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3, ... (17 entities in total)
• 機能のキーワード: transcription, ribosome biogenesis
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Nucleus, nucleolus : P40422 P32529 P22139 P28000 Q01080 P47006 P46669 P10964 P22138 P07703 P50106; Cytoplasm : P20435; Nucleus : P20436 P20434
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 1254194.62

構造登録者

Engel, C.,Sainsbury, S.,Cheung, A.C.,Kostrewa, D.,Cramer, P. (登録日: 2013-08-19, 公開日: 2013-10-23, 最終更新日: 2024-10-23)

主引用文献

Engel, C.,Sainsbury, S.,Cheung, A.C.,Kostrewa, D.,Cramer, P.
RNA Polymerase I Structure and Transcription Regulation.
Nature, 502:502-, 2013

PubMed Abstract: Transcription of ribosomal RNA by RNA polymerase (Pol) I initiates ribosome biogenesis and regulates eukaryotic cell growth. The crystal structure of Pol I from the yeast Saccharomyces cerevisiae at 2.8 Å resolution reveals all 14 subunits of the 590-kilodalton enzyme, and shows differences to Pol II. An 'expander' element occupies the DNA template site and stabilizes an expanded active centre cleft with an unwound bridge helix. A 'connector' element invades the cleft of an adjacent polymerase and stabilizes an inactive polymerase dimer. The connector and expander must detach during Pol I activation to enable transcription initiation and cleft contraction by convergent movement of the polymerase 'core' and 'shelf' modules. Conversion between an inactive expanded and an active contracted polymerase state may generally underlie transcription. Regulatory factors can modulate the core-shelf interface that includes a 'composite' active site for RNA chain initiation, elongation, proofreading and termination.

PubMed: 24153182
DOI: 10.1038/NATURE12712

実験手法

X-RAY DIFFRACTION (2.8 Å)