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4C1F

Crystal structure of the metallo-beta-lactamase IMP-1 with L-captopril

Summary for 4C1F
Entry DOI10.2210/pdb4c1f/pdb
Related4BZ3 4C09 4C1C 4C1D 4C1E 4C1G 4C1H
DescriptorBETA-LACTAMASE IMP-1, L-CAPTOPRIL, SULFATE ION, ... (5 entities in total)
Functional Keywordshydrolase, mbl, metallo-beta-lactamase, antibiotic resistance
Biological sourceSERRATIA MARCESCENS
Cellular locationPeriplasm : P52699
Total number of polymer chains2
Total formula weight51060.46
Authors
Zollman, D.,Brem, J.,McDonough, M.A.,van Berkel, S.S.,Schofield, C.J. (deposition date: 2013-08-12, release date: 2014-08-27, Last modification date: 2023-12-20)
Primary citationBrem, J.,van Berkel, S.S.,Zollman, D.,Lee, S.Y.,Gileadi, O.,McHugh, P.J.,Walsh, T.R.,McDonough, M.A.,Schofield, C.J.
Structural Basis of Metallo-beta-Lactamase Inhibition by Captopril Stereoisomers.
Antimicrob. Agents Chemother., 60:142-150, 2015
Cited by
PubMed Abstract: β-Lactams are the most successful antibacterials, but their effectiveness is threatened by resistance, most importantly by production of serine- and metallo-β-lactamases (MBLs). MBLs are of increasing concern because they catalyze the hydrolysis of almost all β-lactam antibiotics, including recent-generation carbapenems. Clinically useful serine-β-lactamase inhibitors have been developed, but such inhibitors are not available for MBLs. l-Captopril, which is used to treat hypertension via angiotensin-converting enzyme inhibition, has been reported to inhibit MBLs by chelating the active site zinc ions via its thiol(ate). We report systematic studies on B1 MBL inhibition by all four captopril stereoisomers. High-resolution crystal structures of three MBLs (IMP-1, BcII, and VIM-2) in complex with either the l- or d-captopril stereoisomer reveal correlations between the binding mode and inhibition potency. The results will be useful in the design of MBL inhibitors with the breadth of selectivity required for clinical application against carbapenem-resistant Enterobacteriaceae and other organisms causing MBL-mediated resistant infections.
PubMed: 26482303
DOI: 10.1128/AAC.01335-15
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.01 Å)
Structure validation

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数据于2024-11-13公开中

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