4C0U
Cryo-EM reconstruction of enterovirus 71 in complex with a neutralizing antibody E18
Summary for 4C0U
| Entry DOI | 10.2210/pdb4c0u/pdb |
| EMDB information | 2397 |
| Descriptor | VP1, VP2, VP3, ... (5 entities in total) |
| Functional Keywords | virus-immune system complex, virus, pathogen, ev71, virus/immune system |
| Biological source | HUMAN ENTEROVIRUS 71 More |
| Total number of polymer chains | 5 |
| Total formula weight | 124384.11 |
| Authors | Plevka, P.,Perera, R.,Cardosa, J.,Suksatu, A.,Kuhn, R.J.,Rossmann, M.G. (deposition date: 2013-08-08, release date: 2014-02-05, Last modification date: 2024-05-08) |
| Primary citation | Plevka, P.,Lim, P.,Perera, R.,Cardosa, J.,Suksatu, A.,Kuhn, R.J.,Rossmann, M.G. Neutralizing Antibodies Can Initiate Genome Release from Human Enterovirus 71. Proc.Natl.Acad.Sci.USA, 111:2134-, 2014 Cited by PubMed Abstract: Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 °C and 37 °C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles. PubMed: 24469789DOI: 10.1073/PNAS.1320624111 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (10 Å) |
Structure validation
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