4BY7

elongating RNA Polymerase II-Bye1 TLD complex

Summary for 4BY7

• Entry DOI: 10.2210/pdb4by7/pdb
• Related: 4BXX 4BXZ 4BY1
• Descriptor: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5, DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11, ... (19 entities in total)
• Functional Keywords: rna polymerase ii, bye1, transcription
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Total number of polymer chains: 16
• Total formula weight: 548106.06

Authors

Kinkelin, K.,Wozniak, G.G.,Rothbart, S.B.,Lidschreiber, M.,Strahl, B.D.,Cramer, P. (deposition date: 2013-07-18, release date: 2013-09-11, Last modification date: 2023-12-20)

Primary citation

Kinkelin, K.,Wozniak, G.G.,Rothbart, S.B.,Lidschreiber, M.,Strahl, B.D.,Cramer, P.
Structures of RNA polymerase II complexes with Bye1, a chromatin-binding PHF3/DIDO homologue.
Proc. Natl. Acad. Sci. U.S.A., 110:15277-15282, 2013

PubMed Abstract: Bypass of Ess1 (Bye1) is a nuclear protein with a domain resembling the central domain in the transcription elongation factor TFIIS. Here we show that Bye1 binds with its TFIIS-like domain (TLD) to RNA polymerase (Pol) II, and report crystal structures of the Bye1 TLD bound to Pol II and three different Pol II-nucleic acid complexes. Like TFIIS, Bye1 binds with its TLD to the Pol II jaw and funnel. In contrast to TFIIS, however, it neither alters the conformation nor the in vitro functions of Pol II. In vivo, Bye1 is recruited to chromatin via its TLD and occupies the 5'-region of active genes. A plant homeo domain (PHD) in Bye1 binds histone H3 tails with trimethylated lysine 4, and this interaction is enhanced by the presence of neighboring posttranslational modifications (PTMs) that mark active transcription and conversely is impaired by repressive PTMs. We identify putative human homologs of Bye1, the proteins PHD finger protein 3 and death-inducer obliterator, which are both implicated in cancer. These results establish Bye1 as the founding member of a unique family of chromatin transcription factors that link histones with active PTMs to transcribing Pol II.

PubMed: 24003114
DOI: 10.1073/pnas.1311010110

Experimental method

X-RAY DIFFRACTION (3.15 Å)