4BX9

Human Vps33A in complex with a fragment of human Vps16

4BX9 の概要

• エントリーDOI: 10.2210/pdb4bx9/pdb
• 関連するPDBエントリー: 4BX8
• 分子名称: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33A, VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG, FORMIC ACID, ... (6 entities in total)
• 機能のキーワード: protein transport, hops, membrane trafficking, sec1/munc18 proteins, tethering
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 149894.64

構造登録者

Graham, S.C.,Wartosch, L.,Gray, S.R.,Scourfield, E.J.,Deane, J.E.,Luzio, J.P.,Owen, D.J. (登録日: 2013-07-09, 公開日: 2013-07-24, 最終更新日: 2023-12-20)

主引用文献

Graham, S.C.,Wartosch, L.,Gray, S.R.,Scourfield, E.J.,Deane, J.E.,Luzio, J.P.,Owen, D.J.
Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.
Proc. Natl. Acad. Sci. U.S.A., 110:13345-13350, 2013

PubMed Abstract: The multisubunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for late endosome-lysosome and autophagosome-lysosome fusion in mammals. We have determined the crystal structure of the human HOPS subunit Vps33A, confirming its identity as a Sec1/Munc18 family member. We show that HOPS subunit Vps16 recruits Vps33A to the human HOPS complex and that residues 642-736 are necessary and sufficient for this interaction, and we present the crystal structure of Vps33A in complex with Vps16(642-736). Mutations at the binding interface disrupt the Vps33A-Vps16 interaction both in vitro and in cells, preventing recruitment of Vps33A to the HOPS complex. The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes.

PubMed: 23901104
DOI: 10.1073/pnas.1307074110

実験手法

X-RAY DIFFRACTION (2.6 Å)