4BV4

Structure and allostery in Toll-Spatzle recognition

4BV4 の概要

• エントリーDOI: 10.2210/pdb4bv4/pdb
• 分子名称: PROTEIN SPAETZLE C-106, PROTEIN TOLL, VARIABLE LYMPHOCYTE RECEPTOR B CHIMERA, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: cytokine recognition, embryonic development, immune system, innate immunity, leucine-rich repeats, cystine-knot, glycoprotein
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 77017.04

構造登録者

Lewis, M.F.,Arnot, C.J.,Beeston, H.,McCoy, A.,Ashcroft, A.E.,Gay, N.J.,Gangloff, M. (登録日: 2013-06-24, 公開日: 2013-12-04, 最終更新日: 2024-10-09)

主引用文献

Lewis, M.,Arnot, C.J.,Beeston, H.,Mccoy, A.,Ashcroft, A.E.,Gay, N.J.,Gangloff, M.
Cytokine Spatzle Binds to the Drosophila Immunoreceptor Toll with a Neurotrophin-Like Specificity and Couples Receptor Activation.
Proc.Natl.Acad.Sci.USA, 110:20461-, 2013

PubMed Abstract: Drosophila Toll functions in embryonic development and innate immunity and is activated by an endogenous ligand, Spätzle (Spz). The related Toll-like receptors in vertebrates also function in immunity but are activated directly by pathogen-associated molecules such as bacterial endotoxin. Here, we present the crystal structure at 2.35-Å resolution of dimeric Spz bound to a Toll ectodomain encompassing the first 13 leucine-rich repeats. The cystine knot of Spz binds the concave face of the Toll leucine-rich repeat solenoid in an area delineated by N-linked glycans and induces a conformational change. Mutagenesis studies confirm that the interface observed in the crystal structure is relevant for signaling. The asymmetric binding mode of Spz to Toll is similar to that of nerve growth factor (NGF) in complex with the p75 neurotrophin receptor but is distinct from that of microbial ligands bound to the Toll-like receptors. Overall, this study indicates an allosteric signaling mechanism for Toll in which ligand binding to the N terminus induces a conformational change that couples to homodimerization of juxtamembrane structures in the Toll ectodomain C terminus.

PubMed: 24282309
DOI: 10.1073/PNAS.1317002110

実験手法

X-RAY DIFFRACTION (2.35 Å)