4BSW

Heterodimeric Fc Antibody Azymetric Variant 2

4BSW の概要

• エントリーDOI: 10.2210/pdb4bsw/pdb
• 関連するPDBエントリー: 4BSV
• 分子名称: HETERODIMERIC FC ANTIBODY AZYMETRIC VARIANT 2, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: immune system, bispecific, scaffold, antibody engineering, fc domain, igg, igg1, immunoglobulin g, zymeworks inc.
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54716.58

構造登録者

Suits, M.D.L.,Spreter, T.,Cabrera, E.E.,Dixit, S.B.,Lario, P.I.,Poon, D.K.Y.,D'Angelo, I.E.P.,Boulanger, M.J. (登録日: 2013-06-11, 公開日: 2013-08-21, 最終更新日: 2023-12-20)

主引用文献

Von Kreudenstein, T.S.,Escobar-Carbrera, E.,Lario, P.I.,D'Angelo, I.,Brault, K.,Kelly, J.,Durocher, Y.,Baardsnes, J.,Woods, R.J.,Xie, M.H.,Girod, P.,Suits, M.D.L.,Boulanger, M.J.,Poon, D.K.Y.,Ng, G.Y.K.,Dixit, S.B.
Improving Biophysical Properties of a Bispecific Antibody Scaffold to Aid Developability: Quality by Molecular Design.
Mabs, 5:646-, 2013

PubMed Abstract: While the concept of Quality-by-Design is addressed at the upstream and downstream process development stages, we questioned whether there are advantages to addressing the issues of biologics quality early in the design of the molecule based on fundamental biophysical characterization, and thereby reduce complexities in the product development stages. Although limited number of bispecific therapeutics are in clinic, these developments have been plagued with difficulty in producing materials of sufficient quality and quantity for both preclinical and clinical studies. The engineered heterodimeric Fc is an industry-wide favorite scaffold for the design of bispecific protein therapeutics because of its structural, and potentially pharmacokinetic, similarity to the natural antibody. Development of molecules based on this concept, however, is challenged by the presence of potential homodimer contamination and stability loss relative to the natural Fc. We engineered a heterodimeric Fc with high heterodimeric specificity that also retains natural Fc-like biophysical properties, and demonstrate here that use of engineered Fc domains that mirror the natural system translates into an efficient and robust upstream stable cell line selection process as a first step toward a more developable therapeutic.

PubMed: 23924797
DOI: 10.4161/MABS.25632

実験手法

X-RAY DIFFRACTION (2.15 Å)