4BRB

Crystal structure of the integral membrane enzyme DgkA-ref, delta 7

4BRB の概要

• エントリーDOI: 10.2210/pdb4brb/pdb
• 分子名称: DIACYLGLYCEROL KINASE, (2R)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE, ZINC ION, ... (6 entities in total)
• 機能のキーワード: transferase, lipid metabolism, membrane protein
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 88684.86

構造登録者

Li, D.,Caffrey, M. (登録日: 2013-06-04, 公開日: 2014-06-18, 最終更新日: 2023-12-20)

主引用文献

Li, D.,Caffrey, M.
Renaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic.
Sci.Rep., 4:5806-, 2014

PubMed Abstract: Membrane proteins play vital roles in the life of the cell and are important therapeutic targets. Producing them in large quantities, pure and fully functional is a major challenge. Many promising projects end when intractable aggregates or precipitates form. Here we show how such unfolded aggregates can be solubilized and the solution mixed with lipid to spontaneously self-assemble a bicontinuous cubic mesophase into the bilayer of which the protein, in a confined, chaperonin-like environment, reconstitutes with 100% efficiency. The test protein, diacylglycerol kinase, reconstituted in the bilayer of the mesophase, was then crystallized in situ by the in meso or lipid cubic phase method providing an X-ray structure to a resolution of 2.55 Å. This highly efficient, inexpensive, simple and rapid approach should find application wherever properly folded, membrane reconstituted and functional proteins are required where the starting material is a denatured aggregate.

PubMed: 25055873
DOI: 10.1038/SREP05806

実験手法

X-RAY DIFFRACTION (2.55 Å)