4BOS

Structure of OTUD2 OTU domain in complex with Ubiquitin K11-linked peptide

4BOS の概要

• エントリーDOI: 10.2210/pdb4bos/pdb
• 関連するPDBエントリー: 4BOP 4BOQ 4BOU
• 分子名称: UBIQUITIN THIOESTERASE OTU1, POLYUBIQUITIN-C, OTUD2, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 57212.71

構造登録者

Mevissen, T.E.T.,Hospenthal, M.K.,Geurink, P.P.,Elliott, P.R.,Akutsu, M.,Arnaudo, N.,Ekkebus, R.,Kulathu, Y.,Wauer, T.,El Oualid, F.,Freund, S.M.V.,Ovaa, H.,Komander, D. (登録日: 2013-05-22, 公開日: 2013-07-24, 最終更新日: 2023-12-20)

主引用文献

Mevissen, T.E.T.,Hospenthal, M.K.,Geurink, P.P.,Elliott, P.R.,Akutsu, M.,Arnaudo, N.,Ekkebus, R.,Kulathu, Y.,Wauer, T.,El Oualid, F.,Freund, S.M.V.,Ovaa, H.,Komander, D.
Otu Deubiquitinases Reveal Mechanisms of Linkage Specificity and Enable Ubiquitin Chain Restriction Analysis.
Cell(Cambridge,Mass.), 154:169-, 2013

PubMed Abstract: Sixteen ovarian tumor (OTU) family deubiquitinases (DUBs) exist in humans, and most members regulate cell-signaling cascades. Several OTU DUBs were reported to be ubiquitin (Ub) chain linkage specific, but comprehensive analyses are missing, and the underlying mechanisms of linkage specificity are unclear. Using Ub chains of all eight linkage types, we reveal that most human OTU enzymes are linkage specific, preferring one, two, or a defined subset of linkage types, including unstudied atypical Ub chains. Biochemical analysis and five crystal structures of OTU DUBs with or without Ub substrates reveal four mechanisms of linkage specificity. Additional Ub-binding domains, the ubiquitinated sequence in the substrate, and defined S1' and S2 Ub-binding sites on the OTU domain enable OTU DUBs to distinguish linkage types. We introduce Ub chain restriction analysis, in which OTU DUBs are used as restriction enzymes to reveal linkage type and the relative abundance of Ub chains on substrates.

PubMed: 23827681
DOI: 10.1016/J.CELL.2013.05.046

実験手法

X-RAY DIFFRACTION (2.35 Å)