4BOE
Japanin from Rhipicephalus appendiculatus bound to cholesterol: Tetragonal crystal form
Summary for 4BOE
| Entry DOI | 10.2210/pdb4boe/pdb |
| Descriptor | JAPANIN, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, CHOLESTEROL, ... (7 entities in total) |
| Functional Keywords | cholesterol binding protein, tick |
| Biological source | RHIPICEPHALUS APPENDICULATUS (BROWN EAR TICK) |
| Total number of polymer chains | 1 |
| Total formula weight | 20660.58 |
| Authors | Roversi, P.,Johnson, S.,Preston, S.,Austyn, J.M.,Nuttall, P.,Lea, S.M. (deposition date: 2013-05-19, release date: 2014-05-28, Last modification date: 2024-11-06) |
| Primary citation | Roversi, P.,Johnson, S.,Preston, S.G.,Nunn, M.A.,Paesen, G.C.,Austyn, J.M.,Nuttall, P.A.,Lea, S.M. Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks. Sci Rep, 7:16057-16057, 2017 Cited by PubMed Abstract: Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives. PubMed: 29167574DOI: 10.1038/s41598-017-16413-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.24 Å) |
Structure validation
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