4BJA

Globin-like protein Glb-12 from C.elegans

Summary for 4BJA

• Entry DOI: 10.2210/pdb4bja/pdb
• Descriptor: PROTEIN GLB-12, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (5 entities in total)
• Functional Keywords: transport protein
• Biological source: CAENORHABDITIS ELEGANS
• Total number of polymer chains: 1
• Total formula weight: 31615.69

Authors

De Henau, S.,Tilleman, L.,Germani, F.,Pauwels, M.,Vlaeminck, C.,Vanfleteren, J.R.,Bert, W.,Pesce, A.,Nardini, M.,Bolognesi, M.,De Wael, K.,Moens, L.,Dewilde, S.,Braeckman, B.P. (deposition date: 2013-04-17, release date: 2014-05-07, Last modification date: 2024-05-08)

Primary citation

De Henau, S.,Tilleman, L.,Vangheel, M.,Luyckx, E.,Trashin, S.,Pauwels, M.,Germani, F.,Vlaeminck, C.,Vanfleteren, J.R.,Bert, W.,Pesce, A.,Nardini, M.,Bolognesi, M.,De Wael, K.,Moens, L.,Dewilde, S.,Braeckman, B.P.
A Redox Signalling Globin is Essential for Reproduction in Caenorhabditis Elegans.
Nat.Commun., 6:8782-, 2015

PubMed Abstract: Moderate levels of reactive oxygen species (ROS) are now recognized as redox signalling molecules. However, thus far, only mitochondria and NADPH oxidases have been identified as cellular sources of ROS in signalling. Here we identify a globin (GLB-12) that produces superoxide, a type of ROS, which serves as an essential signal for reproduction in C. elegans. We find that GLB-12 has an important role in the regulation of multiple aspects in germline development, including germ cell apoptosis. We further describe how GLB-12 displays specific molecular, biochemical and structural properties that allow this globin to act as a superoxide generator. In addition, both an intra- and extracellular superoxide dismutase act as key partners of GLB-12 to create a transmembrane redox signal. Our results show that a globin can function as a driving factor in redox signalling, and how this signal is regulated at the subcellular level by multiple control layers.

PubMed: 26621324
DOI: 10.1038/NCOMMS9782

Experimental method

X-RAY DIFFRACTION (1.65 Å)