4BHK

Crystal Structure of Moss Leafy bound to DNA

4BHK の概要

• エントリーDOI: 10.2210/pdb4bhk/pdb
• 分子名称: FLORICAULA/LEAFY HOMOLOG 1, MOSS-CR54 DNA, ... (4 entities in total)
• 機能のキーワード: transcription-dna complex, transcription/dna
• 由来する生物種: PHYSCOMITRELLA PATENS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57880.11

構造登録者

Nanao, M.H.,Sayou, C.,Dumas, R.,Parcy, F. (登録日: 2013-04-03, 公開日: 2014-01-22, 最終更新日: 2023-12-20)

主引用文献

Sayou, C.,Monniaux, M.,Nanao, M.H.,Moyroud, E.,Brockington, S.F.,Thevenon, E.,Chahtane, H.,Warthmann, N.,Melkonian, M.,Zhang, Y.,Wong, G.K.,Weigel, D.,Parcy, F.,Dumas, R.
A Promiscuous Intermediate Underlies the Evolution of Leafy DNA Binding Specificity.
Science, 343:645-, 2014

PubMed Abstract: Transcription factors (TFs) are key players in evolution. Changes affecting their function can yield novel life forms but may also have deleterious effects. Consequently, gene duplication events that release one gene copy from selective pressure are thought to be the common mechanism by which TFs acquire new activities. Here, we show that LEAFY, a major regulator of flower development and cell division in land plants, underwent changes to its DNA binding specificity, even though plant genomes generally contain a single copy of the LEAFY gene. We examined how these changes occurred at the structural level and identify an intermediate LEAFY form in hornworts that appears to adopt all different specificities. This promiscuous intermediate could have smoothed the evolutionary transitions, thereby allowing LEAFY to evolve new binding specificities while remaining a single-copy gene.

PubMed: 24436181
DOI: 10.1126/SCIENCE.1248229

実験手法

X-RAY DIFFRACTION (2.32 Å)