4BDB

Fragment-based screening identifies a new area for inhibitor binding to checkpoint kinase 2 (CHK2)

4BDB の概要

• エントリーDOI: 10.2210/pdb4bdb/pdb
• 関連するPDBエントリー: 1GXC 2CN5 2CN8 2W0J 2W7X 2WTC 2WTD 2WTI 2WTJ 2XBJ 2XK9 2XM8 2XM9 2YCF 2YCQ 2YCR 2YCS 2YIQ 2YIR 2YIT 4A9R 4A9S 4A9T 4A9U 4BDA 4BDC 4BDD 4BDE 4BDF 4BDG 4BDH 4BDI 4BDJ 4BDK
• 分子名称: SERINE/THREONINE-PROTEIN KINASE CHK2, NITRATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Isoform 2: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus. Nucleus, PML body: O96017
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37899.62

構造登録者

Silva-Santisteban, M.C.,Westwood, I.M.,Boxall, K.,Brown, N.,Peacock, S.,McAndrew, C.,Barrie, E.,Richards, M.,Mirza, A.,Oliver, A.W.,Burke, R.,Hoelder, S.,Jones, K.,Aherne, G.W.,Blagg, J.,Collins, I.,Garrett, M.D.,van Montfort, R.L.M. (登録日: 2012-10-05, 公開日: 2013-06-26, 最終更新日: 2023-12-20)

主引用文献

Silva-Santisteban, M.C.,Westwood, I.M.,Boxall, K.,Brown, N.,Peacock, S.,Mcandrew, C.,Barrie, E.,Richards, M.,Mirza, A.,Oliver, A.W.,Burke, R.,Hoelder, S.,Jones, K.,Aherne, G.W.,Blagg, J.,Collins, I.,Garrett, M.D.,Van Montfort, R.L.
Fragment-Based Screening Maps Inhibitor Interactions in the ATP-Binding Site of Checkpoint Kinase 2.
Plos One, 8:65689-, 2013

PubMed Abstract: Checkpoint kinase 2 (CHK2) is an important serine/threonine kinase in the cellular response to DNA damage. A fragment-based screening campaign using a combination of a high-concentration AlphaScreen™ kinase assay and a biophysical thermal shift assay, followed by X-ray crystallography, identified a number of chemically different ligand-efficient CHK2 hinge-binding scaffolds that have not been exploited in known CHK2 inhibitors. In addition, it showed that the use of these orthogonal techniques allowed efficient discrimination between genuine hit matter and false positives from each individual assay technology. Furthermore, the CHK2 crystal structures with a quinoxaline-based fragment and its follow-up compound highlight a hydrophobic area above the hinge region not previously explored in rational CHK2 inhibitor design, but which might be exploited to enhance both potency and selectivity of CHK2 inhibitors.

PubMed: 23776527
DOI: 10.1371/JOURNAL.PONE.0065689

実験手法

X-RAY DIFFRACTION (2.5 Å)