4BD3

Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity

Summary for 4BD3

• Entry DOI: 10.2210/pdb4bd3/pdb
• Related: 2B2T 2B2U 2B2V 2B2W 2C1J 2C1N 2CV5 2UXN 3ZVY 4A0J 4A0N 4A7J
• NMR Information: BMRB: 18764
• Descriptor: PHD FINGER PROTEIN 19, HISTONE H3 (2 entities in total)
• Functional Keywords: phf-19, histone recognition, h3k36me3, transcription
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus: Q5T6S3 P68431
• Total number of polymer chains: 2
• Total formula weight: 7981.15

Authors

Lapinaite, A.,Simon, B.,Carlomagno, T. (deposition date: 2012-10-04, release date: 2012-10-31, Last modification date: 2023-06-14)

Primary citation

Ballare, C.,Lange, M.,Lapinaite, A.,Mas Martin, G.,Morey, L.,Pascu, G.,Liefke, R.,Simon, B.,Shi, Y.,Gozani, O.,Carlomagno, T.,Benitah, S.A.,Di Croce, L.
Phf19 Links Methylated Lys36 of Histone H3 to Regulation of Polycomb Activity
Nat.Struct.Mol.Biol., 19:1257-, 2012

PubMed Abstract: Polycomb-group proteins are transcriptional repressors with essential roles in embryonic development. Polycomb repressive complex 2 (PRC2) contains the methyltransferase activity for Lys27. However, the role of other histone modifications in regulating PRC2 activity is just beginning to be understood. Here we show that direct recognition of methylated histone H3 Lys36 (H3K36me), a mark associated with activation, by the PRC2 subunit Phf19 is required for the full enzymatic activity of the PRC2 complex. Using NMR spectroscopy, we provide structural evidence for this interaction. Furthermore, we show that Phf19 binds to a subset of PRC2 targets in mouse embryonic stem cells and that this is required for their repression and for H3K27me3 deposition. These findings show that the interaction of Phf19 with H3K36me2 and H3K36me3 is essential for PRC2 complex activity and for proper regulation of gene repression in embryonic stem cells.

PubMed: 23104054
DOI: 10.1038/NSMB.2434

Experimental method

SOLUTION NMR