4BAG

Feruloyl Esterase Domain of XYNY from Clostridium thermocellum after exposure to 266nm UV laser

Summary for 4BAG

• Entry DOI: 10.2210/pdb4bag/pdb
• Related: 1DYO 1GKK 1GKL 1H6X 1H6Y 1OHZ 1WB4 1WB5 1WB6 2CCL 2W5F 2WYS 2WZE
• Descriptor: ENDO-1,4-BETA-XYLANASE Y, CADMIUM ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: hydrolase
• Biological source: CLOSTRIDIUM THERMOCELLUM
• Total number of polymer chains: 2
• Total formula weight: 69524.51

Authors

Pereira, P.J.B.,de Sanctis, D. (deposition date: 2012-09-14, release date: 2013-04-17, Last modification date: 2024-10-09)

Primary citation

Pereira, P.J.B.,Royant, A.,Panjikar, S.,De Sanctis, D.
In-House Uv Radiation-Damage-Induced Phasing of Selenomethionine-Labeled Protein Structures.
J.Struct.Biol., 181:89-, 2013

PubMed Abstract: Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

PubMed: 23178456
DOI: 10.1016/J.JSB.2012.11.003

Experimental method

X-RAY DIFFRACTION (1.9 Å)