4B8X

Near atomic resolution crystal structure of Sco5413, a MarR family transcriptional regulator from Streptomyces coelicolor

Summary for 4B8X

• Entry DOI: 10.2210/pdb4b8x/pdb
• Descriptor: POSSIBLE MARR-TRANSCRIPTIONAL REGULATOR, CHLORIDE ION (3 entities in total)
• Functional Keywords: transcription, winged helix motif
• Biological source: STREPTOMYCES COELICOLOR
• Total number of polymer chains: 2
• Total formula weight: 31857.48

Authors

Holley, T.A.,Stevenson, C.E.M.,Bibb, M.J.,Lawson, D.M. (deposition date: 2012-08-31, release date: 2012-10-17, Last modification date: 2024-11-06)

Primary citation

Holley, T.A.,Stevenson, C.E.M.,Bibb, M.J.,Lawson, D.M.
High Resolution Crystal Structure of Sco5413, a Widespread Actinomycete Marr Family Transcriptional Regulator of Unknown Function.
Proteins, 81:176-, 2013

PubMed Abstract: The crystal structure of Sco5413 from Streptomyces coelicolor A3(2) has been determined at 1.25 Å resolution, the highest resolution reported for a MarR family transcriptional regulator. Putative orthologs are encoded by the majority of sequenced actinomycete genomes, and may play roles in regulating growth and antibiotic production, but they have yet to be assigned a precise function. Sco5413 forms a homodimer and, through comparisons with other MarR family protein structures, we postulate that it adopts a conformation compatible with DNA binding, and that a channel at the dimer interface, lined by well-conserved residues, is the binding site of an unidentified effector ligand.

PubMed: 23042442
DOI: 10.1002/PROT.24197

Experimental method

X-RAY DIFFRACTION (1.25 Å)