4AEX

HCV-JFH1 NS5B POLYMERASE STRUCTURE AT 2.4 ANGSTROM in a primitive orthorhombic space group

Summary for 4AEX

• Entry DOI: 10.2210/pdb4aex/pdb
• Related: 2XXD 2XYM 4AEP
• Descriptor: RNA-DIRECTED RNA POLYMERASE, PHOSPHATE ION (3 entities in total)
• Functional Keywords: transferase, hepacivirus, nonstructural proteins, replication, de novo initiation, priming
• Biological source: HEPATITIS C VIRUS (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (Probable). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): Q99IB8
• Total number of polymer chains: 2
• Total formula weight: 129357.28

Authors

Caillet-Saguy, C.,Bressanelli, S. (deposition date: 2012-01-12, release date: 2012-05-02, Last modification date: 2023-12-20)

• Primary citation: Scrima, N.,Caillet-Saguy, C.,Ventura, M.,Harrus, D.,Astier-Gin, T.,Bressanelli, S.; Two Crucial Early Steps in RNA Synthesis by the Hepatitis C Virus Polymerase Involve a Dual Role of Residue 405.; J.Virol., 86:7107-, 2012; PubMed: 22532694; DOI: 10.1128/JVI.00459-12

Experimental method

X-RAY DIFFRACTION (2.41 Å)