4ACO
Structure of the budding yeast Ndc10 N-terminal domain
Summary for 4ACO
| Entry DOI | 10.2210/pdb4aco/pdb |
| Descriptor | CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT A (2 entities in total) |
| Functional Keywords | dna binding protein |
| Biological source | SACCHAROMYCES CEREVISIAE |
| Cellular location | Nucleus: P32504 |
| Total number of polymer chains | 1 |
| Total formula weight | 112066.03 |
| Authors | Perriches, T.,Singleton, M.R. (deposition date: 2011-12-16, release date: 2012-01-11, Last modification date: 2024-05-08) |
| Primary citation | Perriches, T.,Singleton, M.R. The Structure of the Yeast Kinetochore Ndc10 DNA-Binding Domain Reveals an Unexpected Evolutionary Relationship to Tyrosine Recombinases. J.Biol.Chem., 287:5173-, 2012 Cited by PubMed Abstract: We have solved the x-ray structure of the N-terminal half of the yeast kinetochore protein Ndc10 at 1.9 Å resolution. This essential protein is a key constituent of the budding yeast centromere and is essential for the recruitment of the centromeric nucleosome and establishment of the kinetochore. The fold of the protein shows unexpected similarities to the tyrosine recombinase/λ-integrase family of proteins, most notably Cre, with some variation in the relative position of the subdomains. This finding offers new insights into kinetochore evolution and the adaptation of a well studied protein fold to a novel role. By comparison with tyrosine recombinases and mutagenesis studies, we have been able to define some of the key DNA-binding motifs. PubMed: 22215672DOI: 10.1074/JBC.C111.318501 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.89 Å) |
Structure validation
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