4AC5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

4AC5 の概要

• エントリーDOI: 10.2210/pdb4ac5/pdb
• 関連するPDBエントリー: 1DXR 1PRC 1R2C 1VRN 2JBL 2PRC 2WJM 2WJN 2X5U 2X5V 3PRC 5PRC 6PRC 7PRC
• 分子名称: PHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, 15-cis-1,2-dihydroneurosporene, REACTION CENTER PROTEIN H CHAIN, ... (10 entities in total)
• 機能のキーワード: photosynthesis, lipidic-sponge phase, reaction center, electron transport, cell membrane, metal- binding, transmembrane, formylation, chromophore, lipoprotein
• 由来する生物種: BLASTOCHLORIS VIRIDIS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 141823.99

構造登録者

Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,DePonte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R. (登録日: 2011-12-14, 公開日: 2012-02-15, 最終更新日: 2023-12-20)

主引用文献

Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,Deponte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R.
Lipidic Phase Membrane Protein Serial Femtosecond Crystallography.
Nat.Methods, 9:263-, 2012

PubMed Abstract: X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

PubMed: 22286383
DOI: 10.1038/NMETH.1867

実験手法

X-RAY DIFFRACTION (8.2 Å)