4AB1

Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers

4AB1 の概要

• エントリーDOI: 10.2210/pdb4ab1/pdb
• 関連するPDBエントリー: 1MX1 1MX5 1MX9 1YA4 1YA8 1YAH 1YAJ
• 分子名称: LIVER CARBOXYLESTERASE 1, 2-acetamido-2-deoxy-beta-D-glucopyranose, THIOCYANATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, whole insect expression systems
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59067.68

構造登録者

Greenblatt, H.M.,Otto, T.C.,Cerasoli, D.M.,Sussman, J.L. (登録日: 2011-12-06, 公開日: 2012-03-07, 最終更新日: 2024-10-23)

主引用文献

Greenblatt, H.M.,Otto, T.C.,Kirkpatrick, M.G.,Kovaleva, E.,Brown, S.,Buchman, G.,Cerasoli, D.M.,Sussman, J.L.
Structure of Recombinant Human Carboxylesterase 1 Isolated from Whole Cabbage Looper Larvae.
Acta Crystallogr.,Sect.F, 68:269-, 2012

PubMed Abstract: The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies.

PubMed: 22442219
DOI: 10.1107/S1744309112003326

実験手法

X-RAY DIFFRACTION (2.2 Å)