4A2I

Cryo-electron Microscopy Structure of the 30S Subunit in Complex with the YjeQ Biogenesis Factor

4A2I の概要

• エントリーDOI: 10.2210/pdb4a2i/pdb
• 関連するPDBエントリー: 1M5G 1MJ1 1P6G 1P87 1S03 1ZN1 2AVY 2AW7 2RCN 2VHO 2VHP 2WWL
• EMDBエントリー: 1895
• 分子名称: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (22 entities in total)
• 機能のキーワード: ribosome-hydrolase complex, ribosome assembly, ribosome/hydrolase
• 由来する生物種: SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; 詳細
• 細胞内の位置: Cytoplasm : Q8ZKB0
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 791447.67

構造登録者

Jomaa, A.,Stewart, G.,Mears, J.A.,Kireeva, I.,Brown, E.D.,Ortega, J. (登録日: 2011-09-27, 公開日: 2011-11-02, 最終更新日: 2024-05-08)

主引用文献

Jomaa, A.,Stewart, G.,Mears, J.A.,Kireeva, I.,Brown, E.D.,Ortega, J.
Cryo-Electron Microscopy Structure of the 30S Subunit in Complex with the Yjeq Biogenesis Factor.
RNA, 17:2026-, 2011

PubMed Abstract: YjeQ is a protein broadly conserved in bacteria containing an N-terminal oligonucleotide/oligosaccharide fold (OB-fold) domain, a central GTPase domain, and a C-terminal zinc-finger domain. YjeQ binds tightly and stoichiometrically to the 30S subunit, which stimulates its GTPase activity by 160-fold. Despite growing evidence for the involvement of the YjeQ protein in bacterial 30S subunit assembly, the specific function and mechanism of this protein remain unclear. Here, we report the costructure of YjeQ with the 30S subunit obtained by cryo-electron microscopy. The costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit. This binding location of YjeQ in the 30S subunit suggests a chaperone role in processing of the 3' end of the rRNA as well as in mediating the correct orientation of the main domains of the 30S subunit. In addition, the YjeQ binding site partially overlaps with the interaction site of initiation factors 2 and 3, and upon binding, YjeQ covers three inter-subunit bridges that are important for the association of the 30S and 50S subunits. Hence, our structure suggests that YjeQ may assist in ribosome maturation by preventing premature formation of the translation initiation complex and association with the 50S subunit. Together, these results support a role for YjeQ in the late stages of 30S maturation.

PubMed: 21960487
DOI: 10.1261/RNA.2922311

実験手法

ELECTRON MICROSCOPY (16.5 Å)