4WA2

The crystal structure of hemagglutinin from a H3N8 influenza virus isolated from New England harbor seals in complex with 3'SLN

Summary for 4WA2

• Entry DOI: 10.2210/pdb4wa2/pdb
• Related: 4WA1 4WA3 4WA4 4WA5
• Descriptor: Hemagglutinin, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: hemagglutinin, influenza virus, seal, viral protein
• Biological source: Influenza A virus (A/harbor seal/Massachusetts/1/2011(H3N8))
• Total number of polymer chains: 6
• Total formula weight: 343009.85

Authors

Yang, H.,Villanueva, J.M.,Gubareva, L.V.,Stevens, J. (deposition date: 2014-08-28, release date: 2015-01-14, Last modification date: 2024-11-13)

Primary citation

Yang, H.,Nguyen, H.T.,Carney, P.J.,Guo, Z.,Chang, J.C.,Jones, J.,Davis, C.T.,Villanueva, J.M.,Gubareva, L.V.,Stevens, J.
Structural and Functional Analysis of Surface Proteins from an A(H3N8) Influenza Virus Isolated from New England Harbor Seals.
J.Virol., 89:2801-2812, 2015

PubMed Abstract: In late 2011, an A(H3N8) influenza virus infection resulted in the deaths of 162 New England harbor seals. Virus sequence analysis and virus receptor binding studies highlighted potential markers responsible for mammalian adaptation and a mixed receptor binding preference (S. J. Anthony, J. A. St Leger, K. Pugliares, H. S. Ip, J. M. Chan, Z. W. Carpenter, I. Navarrete-Macias, M. Sanchez-Leon, J. T. Saliki, J. Pedersen, W. Karesh, P. Daszak, R. Rabadan, T. Rowles, W. I. Lipkin, MBio 3:e00166-00112, 2012, http://dx.doi.org/10.1128/mBio.00166-12). Here, we present a detailed structural and biochemical analysis of the surface antigens of the virus. Results obtained with recombinant proteins for both the hemagglutinin and neuraminidase indicate a true avian receptor binding preference. Although the detection of this virus in new species highlights an increased potential for cross-species transmission, our results indicate that the A(H3N8) virus currently poses a low risk to humans.

PubMed: 25540377
DOI: 10.1128/JVI.02723-14

Experimental method

X-RAY DIFFRACTION (2.5 Å)