4UOI
Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1
Summary for 4UOI
| Entry DOI | 10.2210/pdb4uoi/pdb |
| Descriptor | GENOME POLYPROTEIN, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total) |
| Functional Keywords | viral protein |
| Biological source | HEPATITIS C VIRUS |
| Total number of polymer chains | 6 |
| Total formula weight | 59912.51 |
| Authors | El Omari, K.,Iourin, O.,Kadlec, J.,Harlos, K.,Grimes, J.M.,Stuart, D.I. (deposition date: 2014-06-04, release date: 2014-08-20, Last modification date: 2024-11-20) |
| Primary citation | El Omari, K.,Iourin, O.,Kadlec, J.,Fearn, R.,Hall, D.R.,Harlos, K.,Grimes, J.M.,Stuart, D.I. Unexpected Structure for the N-Terminal Domain of Hepatitis C Virus Envelope Glycoprotein E1 Acta Crystallogr.,Sect.D, 70:2197-, 2014 Cited by PubMed Abstract: Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 Å resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 Å resolution model building was achievable. PubMed: 25084338DOI: 10.1107/S139900471401339X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.49 Å) |
Structure validation
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