4UOI
Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.006, 105.006, 204.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.290 - 3.490 |
R-factor | 0.2144 |
Rwork | 0.213 |
R-free | 0.23970 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.140 | 0.810 |
Number of reflections | 15137 | |
<I/σ(I)> | 17.6 | 2.2 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.8 | 15% (WT/V) PEG 1500, 3.6% (WT/V) PEG 4000 AND 0.05 M SODIUM ACETATE PH 4.8 |