4UOI
Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 105.006, 105.006, 204.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.290 - 3.490 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.23970 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.140 | 0.810 |
| Number of reflections | 15137 | |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 15% (WT/V) PEG 1500, 3.6% (WT/V) PEG 4000 AND 0.05 M SODIUM ACETATE PH 4.8 |
