4TPH

Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2

Summary for 4TPH

• Entry DOI: 10.2210/pdb4tph/pdb
• Related: 4tpg 4tpj
• Descriptor: Proton:oligopeptide symporter POT family, ZINC ION, DODECYL-BETA-D-MALTOSIDE, ... (5 entities in total)
• Functional Keywords: membrane protein, secondary active transporter, complex
• Biological source: Shewanella oneidensis
• Total number of polymer chains: 2
• Total formula weight: 116473.63

Authors

Guettou, F.,Quistgaard, E.,Raba, M.,Moberg, P.,Low, C.,Nordlund, P. (deposition date: 2014-06-07, release date: 2014-07-09, Last modification date: 2023-12-20)

Primary citation

Guettou, F.,Quistgaard, E.M.,Raba, M.,Moberg, P.,Low, C.,Nordlund, P.
Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.
Nat.Struct.Mol.Biol., 21:728-, 2014

PubMed Abstract: Peptide transporters of the PepT family have key roles in the transport of di- and tripeptides across membranes as well as in the absorption of orally administered drugs in the small intestine. We have determined structures of a PepT transporter from Shewanella oneidensis (PepT(So2)) in complex with three different peptides. The peptides bind in a large cavity lined by residues that are highly conserved in human PepT1 and PepT2. The bound peptides adopt extended conformations with their N termini clamped into a conserved polar pocket. A positively charged patch allows differential interactions with the C-terminal carboxylates of di- and tripeptides. Here we identify three pockets for peptide side chain interactions, and our binding studies define differential roles of these pockets for the recognition of different subtypes of peptide side chains.

PubMed: 25064511
DOI: 10.1038/nsmb.2860

Experimental method

X-RAY DIFFRACTION (3.155 Å)