4TPH
Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-04-20 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.650, 107.680, 204.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.543 - 3.155 |
R-factor | 0.2537 |
Rwork | 0.251 |
R-free | 0.30730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lep |
RMSD bond length | 0.013 |
RMSD bond angle | 1.794 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 3.240 |
High resolution limit [Å] | 3.160 | 3.160 |
Number of reflections | 24515 | |
<I/σ(I)> | 14.06 | |
Completeness [%] | 77.5 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 273 | 0.1 M phosphate citrate pH 4.0, 36% PEG300, 0.12 M ZnCl2 |