4OF3

Crystal Structure of SYG-1 D1-D2, Glycosylated

Summary for 4OF3

• Entry DOI: 10.2210/pdb4of3/pdb
• Related: 4OF0 4OF6 4OF7 4OF8 4OFD 4OFI 4OFK 4OFP 4OFY
• Descriptor: Protein SYG-1, isoform b, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: immunoglobulin superfamily, cell adhesion, synaptogenesis, protein binding, n-linked glycosylation, membrane, extracellular, signaling protein
• Biological source: Caenorhabditis elegans (nematode)
• Total number of polymer chains: 2
• Total formula weight: 62624.31

Authors

Ozkan, E.,Garcia, K.C. (deposition date: 2014-01-14, release date: 2014-02-19, Last modification date: 2023-09-20)

Primary citation

Ozkan, E.,Chia, P.H.,Wang, R.R.,Goriatcheva, N.,Borek, D.,Otwinowski, Z.,Walz, T.,Shen, K.,Garcia, K.C.
Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis.
Cell(Cambridge,Mass.), 156:482-494, 2014

PubMed Abstract: SYG-1 and SYG-2 are multipurpose cell adhesion molecules (CAMs) that have evolved across all major animal taxa to participate in diverse physiological functions, ranging from synapse formation to formation of the kidney filtration barrier. In the crystal structures of several SYG-1 and SYG-2 orthologs and their complexes, we find that SYG-1 orthologs homodimerize through a common, bispecific interface that similarly mediates an unusual orthogonal docking geometry in the heterophilic SYG-1/SYG-2 complex. C. elegans SYG-1's specification of proper synapse formation in vivo closely correlates with the heterophilic complex affinity, which appears to be tuned for optimal function. Furthermore, replacement of the interacting domains of SYG-1 and SYG-2 with those from CAM complexes that assume alternative docking geometries or the introduction of segmental flexibility compromised synaptic function. These results suggest that SYG extracellular complexes do not simply act as "molecular velcro" and that their distinct structural features are important in instructing synaptogenesis. PAPERFLICK:

PubMed: 24485456
DOI: 10.1016/j.cell.2014.01.004

Experimental method

X-RAY DIFFRACTION (2.5 Å)