4OF3

Crystal Structure of SYG-1 D1-D2, Glycosylated

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Protein SYG-1, isoform b	polymer	274	30375.3	2	UniProt (B1Q236) Pfam (PF07679) Pfam (PF08205) In PDB	Caenorhabditis elegans (nematode)
2	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
3	D	alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		716.7	1	In PDB GlyTouCan (G63564LA)
4	E	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5		water	water		18.0	39	Chemie (HOH)

Sequence modifications

A, B: 19 - 282 (UniProt: B1Q236)

PDB	External Database	Details
Ala 16	-	expression tag
Asp 17	-	expression tag
Pro 18	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60750.5
Branched	Number of molecules	3
	Total formula weight	1873.8
All*	Total formula weight	62624.3

*Water molecules are not included.