4L4V

Structure of human MAIT TCR in complex with human MR1-RL-6-Me-7-OH

Summary for 4L4V

• Entry DOI: 10.2210/pdb4l4v/pdb
• Related: 4GUP 4L4T
• Descriptor: Major histocompatibility complex class I-related gene protein, Beta-2-microglobulin, MAIT T-cell receptor alpha chain, ... (7 entities in total)
• Functional Keywords: mhc class i-related protein, mait tcr, immune system, vitamin b metabolites, membrane protein-immune system complex, membrane protein/immune system
• Biological source: Homo sapiens (human); More
• Cellular location: Cell membrane; Single-pass membrane protein; Extracellular side. Isoform 4: Secreted: Q95460; Secreted: P61769
• Total number of polymer chains: 8
• Total formula weight: 188245.77

Authors

Patel, O.,Kjer-Nielsen, L.,Le Nours, J.,Eckle, S.B.G.,Birkinshaw, R.W.,Beddoe, T.,Corbett, A.J.,Liu, L.,Miles, J.J.,Meehan, B.,Reantragoon, R.,Sandoval-Romero, M.L.,Sullivan, L.C.,Brooks, A.G.,Chen, Z.,Fairlie, D.P.,McCluskey, J.,Rossjohn, J. (deposition date: 2013-06-09, release date: 2013-07-17, Last modification date: 2024-11-20)

Primary citation

Patel, O.,Kjer-Nielsen, L.,Le Nours, J.,Eckle, S.B.,Birkinshaw, R.,Beddoe, T.,Corbett, A.J.,Liu, L.,Miles, J.J.,Meehan, B.,Reantragoon, R.,Sandoval-Romero, M.L.,Sullivan, L.C.,Brooks, A.G.,Chen, Z.,Fairlie, D.P.,McCluskey, J.,Rossjohn, J.
Recognition of vitamin B metabolites by mucosal-associated invariant T cells.
Nat Commun, 4:2142-2142, 2013

PubMed Abstract: The mucosal-associated invariant T-cell antigen receptor (MAIT TCR) recognizes MR1 presenting vitamin B metabolites. Here we describe the structures of a human MAIT TCR in complex with human MR1 presenting a non-stimulatory ligand derived from folic acid and an agonist ligand derived from a riboflavin metabolite. For both vitamin B antigens, the MAIT TCR docks in a conserved manner above MR1, thus acting as an innate-like pattern recognition receptor. The invariant MAIT TCR α-chain usage is attributable to MR1-mediated interactions that prise open the MR1 cleft to allow contact with the vitamin B metabolite. Although the non-stimulatory antigen does not contact the MAIT TCR, the stimulatory antigen does. This results in a higher affinity of the MAIT TCR for a stimulatory antigen in comparison with a non-stimulatory antigen. We formally demonstrate a structural basis for MAIT TCR recognition of vitamin B metabolites, while illuminating how TCRs recognize microbial metabolic signatures.

PubMed: 23846752
DOI: 10.1038/ncomms3142

Experimental method

X-RAY DIFFRACTION (1.9 Å)