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4L4V

Structure of human MAIT TCR in complex with human MR1-RL-6-Me-7-OH

Summary for 4L4V
Entry DOI10.2210/pdb4l4v/pdb
Related4GUP 4L4T
DescriptorMajor histocompatibility complex class I-related gene protein, Beta-2-microglobulin, MAIT T-cell receptor alpha chain, ... (7 entities in total)
Functional Keywordsmhc class i-related protein, mait tcr, immune system, vitamin b metabolites, membrane protein-immune system complex, membrane protein/immune system
Biological sourceHomo sapiens (human)
More
Cellular locationCell membrane; Single-pass membrane protein; Extracellular side. Isoform 4: Secreted: Q95460
Secreted: P61769
Total number of polymer chains8
Total formula weight188245.77
Authors
Primary citationPatel, O.,Kjer-Nielsen, L.,Le Nours, J.,Eckle, S.B.,Birkinshaw, R.,Beddoe, T.,Corbett, A.J.,Liu, L.,Miles, J.J.,Meehan, B.,Reantragoon, R.,Sandoval-Romero, M.L.,Sullivan, L.C.,Brooks, A.G.,Chen, Z.,Fairlie, D.P.,McCluskey, J.,Rossjohn, J.
Recognition of vitamin B metabolites by mucosal-associated invariant T cells.
Nat Commun, 4:2142-2142, 2013
Cited by
PubMed Abstract: The mucosal-associated invariant T-cell antigen receptor (MAIT TCR) recognizes MR1 presenting vitamin B metabolites. Here we describe the structures of a human MAIT TCR in complex with human MR1 presenting a non-stimulatory ligand derived from folic acid and an agonist ligand derived from a riboflavin metabolite. For both vitamin B antigens, the MAIT TCR docks in a conserved manner above MR1, thus acting as an innate-like pattern recognition receptor. The invariant MAIT TCR α-chain usage is attributable to MR1-mediated interactions that prise open the MR1 cleft to allow contact with the vitamin B metabolite. Although the non-stimulatory antigen does not contact the MAIT TCR, the stimulatory antigen does. This results in a higher affinity of the MAIT TCR for a stimulatory antigen in comparison with a non-stimulatory antigen. We formally demonstrate a structural basis for MAIT TCR recognition of vitamin B metabolites, while illuminating how TCRs recognize microbial metabolic signatures.
PubMed: 23846752
DOI: 10.1038/ncomms3142
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.9 Å)
Structure validation

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