4L4V
Structure of human MAIT TCR in complex with human MR1-RL-6-Me-7-OH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.370, 71.203, 144.642 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 40.450 - 1.900 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.20910 |
| Structure solution method | PHASER |
| Starting model (for MR) | 4gup |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.440 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.189 | 1.260 |
| Number of reflections | 168903 | |
| <I/σ(I)> | 9.8 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
