4JGF
Crystal Structure of the Cataract-Causing P23T gamma D-Crystallin Mutant
Summary for 4JGF
Entry DOI | 10.2210/pdb4jgf/pdb |
Descriptor | Gamma-crystallin D (2 entities in total) |
Functional Keywords | structural protein |
Biological source | Homo sapiens (human) |
Total number of polymer chains | 2 |
Total formula weight | 40809.41 |
Authors | Ji, F.L.,Koharudin, L.M.,Jung, J.W.,Gronenborn, A.M. (deposition date: 2013-03-01, release date: 2013-05-22, Last modification date: 2024-02-28) |
Primary citation | Ji, F.,Koharudin, L.M.,Jung, J.,Gronenborn, A.M. Crystal structure of the cataract-causing P23T gamma D-crystallin mutant. Proteins, 81:1493-1498, 2013 Cited by PubMed Abstract: Up to now, efforts to crystallize the cataract-associated P23T mutant of human γD-crystallin have not been successful. Therefore, insights into the light scattering mechanism of this mutant have been exclusively obtained from solution work. Here we present the first crystal structure of the P23T mutant at 2.5 Å resolution. The protein exhibits essentially the same overall structure as seen for the wild-type protein. Based on our structural data, we confirm that no major conformational changes are caused by the mutation, and that solution phase properties of the mutant appear exclusively associated with cataract formation. PubMed: 23670788DOI: 10.1002/prot.24321 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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