4DID
Crystal structure of Salmonella effector N-terminal domain SopB in complex with Cdc42
Summary for 4DID
| Entry DOI | 10.2210/pdb4did/pdb |
| Descriptor | Cell division control protein 42 homolog, Inositol phosphate phosphatase sopB, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total) |
| Functional Keywords | small gtpase, gtp binding, hydrolase-hydrolase complex, hydrolase/hydrolase |
| Biological source | Homo sapiens (human) More |
| Cellular location | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P60953 Secreted (By similarity): O30916 |
| Total number of polymer chains | 2 |
| Total formula weight | 38764.26 |
| Authors | Burkinshaw, B.J.,Strynadka, N.C.J. (deposition date: 2012-01-30, release date: 2012-02-29, Last modification date: 2023-09-13) |
| Primary citation | Burkinshaw, B.J.,Prehna, G.,Worrall, L.J.,Strynadka, N.C. Structure of Salmonella Effector Protein SopB N-terminal Domain in Complex with Host Rho GTPase Cdc42. J.Biol.Chem., 287:13348-13355, 2012 Cited by PubMed Abstract: SopB is a type III secreted Salmonella effector protein with phosphoinositide phosphatase activity and a distinct GTPase binding domain. The latter interacts with host Cdc42, an essential Rho GTPase that regulates critical events in eukaryotic cytoskeleton organization and membrane trafficking. Structural and biochemical analysis of the SopB GTPase binding domain in complex with Cdc42 shows for the first time that SopB structurally and functionally mimics a host guanine nucleotide dissociation inhibitor (GDI) by contacting key residues in the regulatory switch regions of Cdc42 and slowing Cdc42 nucleotide exchange. PubMed: 22362774DOI: 10.1074/jbc.M111.331330 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3501 Å) |
Structure validation
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