4DID

Crystal structure of Salmonella effector N-terminal domain SopB in complex with Cdc42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	200
Detector technology	CCD
Collection date	2010-07-29
Detector	RAYONIX MX300HE
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.880, 106.880, 87.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.617 - 2.350
R-factor	0.2085
Rwork	0.207
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1an0
RMSD bond length	0.010
RMSD bond angle	1.141
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_893))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.450
High resolution limit [Å]	2.350	2.350
Number of reflections	21675
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	277	0.2M sodium chloride, 0.1M phosphate-citrate pH 4.2, 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K