3ZVC

3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82

3ZVC の概要

• エントリーDOI: 10.2210/pdb3zvc/pdb
• 関連するPDBエントリー: 3ZV8 3ZV9 3ZVA 3ZVB 3ZVD 3ZVE 3ZVF 3ZVG
• 分子名称: 3C PROTEASE, ETHYL (5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE (3 entities in total)
• 機能のキーワード: hydrolase, michael inhibitor
• 由来する生物種: HUMAN ENTEROVIRUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21857.83

構造登録者

Tan, J.,Perbandt, M.,Mesters, J.R.,Hilgenfeld, R. (登録日: 2011-07-24, 公開日: 2012-08-08, 最終更新日: 2024-10-23)

主引用文献

Tan, J.,George, S.,Kusov, Y.,Perbandt, M.,Anemuller, S.,Mesters, J.R.,Norder, H.,Coutard, B.,Lacroix, C.,Leyssen, P.,Neyts, J.,Hilgenfeld, R.
3C Protease of Enterovirus 68: Structure-Based Design of Michael Acceptor Inhibitors and Their Broad-Spectrum Antiviral Effects Against Picornaviruses.
J.Virol., 87:4339-, 2013

PubMed Abstract: We have determined the cleavage specificity and the crystal structure of the 3C protease of enterovirus 68 (EV68 3C(pro)). The protease exhibits a typical chymotrypsin fold with a Cys...His...Glu catalytic triad; its three-dimensional structure is closely related to that of the 3C(pro) of rhinovirus 2, as well as to that of poliovirus. The phylogenetic position of the EV68 3C(pro) between the corresponding enzymes of rhinoviruses on the one hand and classical enteroviruses on the other prompted us to use the crystal structure for the design of irreversible inhibitors, with the goal of discovering broad-spectrum antiviral compounds. We synthesized a series of peptidic α,β-unsaturated ethyl esters of increasing length and for each inhibitor candidate, we determined a crystal structure of its complex with the EV68 3C(pro), which served as the basis for the next design round. To exhibit inhibitory activity, compounds must span at least P3 to P1'; the most potent inhibitors comprise P4 to P1'. Inhibitory activities were found against the purified 3C protease of EV68, as well as with replicons for poliovirus and EV71 (50% effective concentration [EC(50)] = 0.5 μM for the best compound). Antiviral activities were determined using cell cultures infected with EV71, poliovirus, echovirus 11, and various rhinovirus serotypes. The most potent inhibitor, SG85, exhibited activity with EC(50)s of ≈180 nM against EV71 and ≈60 nM against human rhinovirus 14 in a live virus-cell-based assay. Even the shorter SG75, spanning only P3 to P1', displayed significant activity (EC(50) = 2 to 5 μM) against various rhinoviruses.

PubMed: 23388726
DOI: 10.1128/JVI.01123-12

実験手法

X-RAY DIFFRACTION (2 Å)