3ZQ6

ADP-ALF4 COMPLEX OF M. THERM. TRC40

Summary for 3ZQ6

• Entry DOI: 10.2210/pdb3zq6/pdb
• Descriptor: PUTATIVE ARSENICAL PUMP-DRIVING ATPASE, ADENOSINE-5'-DIPHOSPHATE, TETRAFLUOROALUMINATE ION, ... (7 entities in total)
• Functional Keywords: tail-anchored, membrane protein, targeting factor atp-binding, arsa, nucleotide-binding, hydrolase
• Biological source: METHANOTHERMOBACTER THERMAUTOTROPHICUS
• Total number of polymer chains: 4
• Total formula weight: 148860.67

Authors

Sherrill, J.,Mariappan, M.,Dominik, P.,Hegde, R.S.,Keenan, R.J. (deposition date: 2011-06-08, release date: 2011-06-22, Last modification date: 2023-12-20)

Primary citation

Sherrill, J.,Mariappan, M.,Dominik, P.,Hegde, R.S.,Keenan, R.J.
A Conserved Archaeal Pathway for Tail-Anchored Membrane Protein Insertion.
Traffic, 12:1119-, 2011

PubMed Abstract: Eukaryotic tail-anchored (TA) membrane proteins are inserted into the endoplasmic reticulum by a post-translational TRC40 pathway, but no comparable pathway is known in other domains of life. The crystal structure of an archaebacterial TRC40 sequence homolog bound to ADP•AlF(4) (-) reveals characteristic features of eukaryotic TRC40, including a zinc-mediated dimer and a large hydrophobic groove. Moreover, archaeal TRC40 interacts with the transmembrane domain of TA substrates and directs their membrane insertion. Thus, the TRC40 pathway is more broadly conserved than previously recognized.

PubMed: 21658170
DOI: 10.1111/J.1600-0854.2011.01229.X

Experimental method

X-RAY DIFFRACTION (2.107 Å)