3ZGO

Re-refined structure of the human Sirt2 apoform

3ZGO の概要

• エントリーDOI: 10.2210/pdb3zgo/pdb
• 関連するPDBエントリー: 3ZGV
• 分子名称: NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2, ZINC ION, HEXAETHYLENE GLYCOL, ... (7 entities in total)
• 機能のキーワード: hydrolase, nad+-dependent deacetylase, sirtuin
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 111618.28

構造登録者

Moniot, S.,Steegborn, C. (登録日: 2012-12-18, 公開日: 2013-03-20, 最終更新日: 2024-11-06)

主引用文献

Moniot, S.,Schutkowski, M.,Steegborn, C.
Crystal Structure Analysis of Human Sirt2 and its Adp-Ribose Complex
J.Struct.Biol., 182:136-, 2013

PubMed Abstract: Sirtuins are NAD(+)-dependent protein deacetylases that regulate metabolism and aging-related processes. Sirt2 is the only cytoplasmic isoform among the seven mamalian Sirtuins (Sirt1-7) and structural information concerning this isoform is limited. We crystallized Sirt2 in complex with a product analog, ADP-ribose, and solved this first crystal structure of a Sirt2 ligand complex at 2.3Å resolution. Additionally, we re-refined the structure of the Sirt2 apoform and analyzed the conformational changes associated with ligand binding to derive insights into the dynamics of the enzyme. Our analyses also provide information on Sirt2 peptide substrate binding and structural states of a Sirt2-specific protein region, and our insights and the novel Sirt2 crystal form provide helpful tools for the development of Sirt2 specific inhibitors.

PubMed: 23454361
DOI: 10.1016/J.JSB.2013.02.012

実験手法

X-RAY DIFFRACTION (1.63 Å)