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3ZEF

Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution

Summary for 3ZEF
Entry DOI10.2210/pdb3zef/pdb
Related4I43
DescriptorA1 CISTRON-SPLICING FACTOR AAR2, PRE-MRNA-SPLICING FACTOR 8 (2 entities in total)
Functional Keywordstranslation, pre-mrna splicing, spliceosome, u5 snrnp
Biological sourceSACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
More
Cellular locationCytoplasm: P32357
Nucleus: P33334
Total number of polymer chains4
Total formula weight436734.00
Authors
Galej, W.P.,Oubridge, C.,Newman, A.J.,Nagai, K. (deposition date: 2012-12-05, release date: 2013-01-30, Last modification date: 2023-12-20)
Primary citationGalej, W.P.,Oubridge, C.,Newman, A.J.,Nagai, K.
Crystal Structure of Prp8 Reveals Active Site Cavity of the Spliceosome
Nature, 493:638-, 2013
Cited by
PubMed Abstract: The active centre of the spliceosome consists of an intricate network formed by U5, U2 and U6 small nuclear RNAs, and a pre-messenger-RNA substrate. Prp8, a component of the U5 small nuclear ribonucleoprotein particle, crosslinks extensively with this RNA catalytic core. Here we present the crystal structure of yeast Prp8 (residues 885-2413) in complex with Aar2, a U5 small nuclear ribonucleoprotein particle assembly factor. The structure reveals tightly associated domains of Prp8 resembling a bacterial group II intron reverse transcriptase and a type II restriction endonuclease. Suppressors of splice-site mutations, and an intron branch-point crosslink, map to a large cavity formed by the reverse transcriptase thumb, and the endonuclease-like and RNaseH-like domains. This cavity is large enough to accommodate the catalytic core of group II intron RNA. The structure provides crucial insights into the architecture of the spliceosome active site, and reinforces the notion that nuclear pre-mRNA splicing and group II intron splicing have a common origin.
PubMed: 23354046
DOI: 10.1038/NATURE11843
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.1 Å)
Structure validation

227344

數據於2024-11-13公開中

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