3ZDW

Substrate and dioxygen binding to the endospore coat laccase CotA from Bacillus subtilis

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3ZDW の概要

• エントリーDOI: 10.2210/pdb3zdw/pdb
• 分子名称: COTA LACCASE, COPPER (I) ION, OXYGEN MOLECULE, ... (6 entities in total)
• 機能のキーワード: hydrolase, abts
• 由来する生物種: BACILLUS SUBTILIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60020.25

構造登録者

Enguita, F.J.,Marcal, D.,Grenha, R.,Lindley, P.F.,Carrondo, M.A. (登録日: 2012-12-01, 公開日: 2012-12-19, 最終更新日: 2024-10-16)

主引用文献

Enguita, F.J.,Marcal, D.,Martins, L.O.,Grenha, R.,Henriques, A.O.,Lindley, P.F.,Carrondo, M.A.
Substrate and Dioxygen Binding to the Endospore Coat Laccase from Bacillus Subtilis.
J.Biol.Chem., 279:23472-, 2004

PubMed Abstract: The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center.

PubMed: 14764581
DOI: 10.1074/JBC.M314000200

実験手法

X-RAY DIFFRACTION (2.4 Å)