3X1Z

Ras-related protein Rap1B(T65A) with GppNHp

3X1Z の概要

• エントリーDOI: 10.2210/pdb3x1z/pdb
• 関連するPDBエントリー: 3W1W 3W1X 3W1Y
• 分子名称: Ras-related protein Rap-1b, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: signal transduction, signaling protein
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• 細胞内の位置: Cell membrane : Q62636
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39112.01

構造登録者

Noguchi, H.,Ikegami, T.,Park, S.Y.,Tame, J.R.H.,Unzai, S. (登録日: 2014-12-02, 公開日: 2015-06-03, 最終更新日: 2024-03-20)

主引用文献

Noguchi, H.,Ikegami, T.,Nagadoi, A.,Kamatari, Y.O.,Park, S.Y.,Tame, J.R.,Unzai, S.
The structure and conformational switching of Rap1B
Biochem.Biophys.Res.Commun., 462:46-51, 2015

PubMed Abstract: Rap1B is a small GTPase involved in the regulation of numerous cellular processes including synaptic plasticity, one of the bases of memory. Like other members of the Ras family, the active GTP-bound form of Rap1B can bind to a large number of effector proteins and so transmit signals to downstream components of the signaling pathways. The structure of Rap1B bound only to a nucleotide has yet to be solved, but might help reveal an inactive conformation that can be stabilized by a small molecule drug. Unlike other Ras family proteins such as H-Ras and Rap2A, Rap1B crystallizes in an intermediate state when bound to a non-hydrolyzable GTP analog. Comparison with H-Ras and Rap2A reveals conservative mutations relative to Rap1B, distant from the bound nucleotide, which control how readily the protein may adopt the fully activated form in the presence of GTP. High resolution crystallographic structures of mutant proteins show how these changes may influence the hydrogen bonding patterns of the key switch residues.

PubMed: 25935485
DOI: 10.1016/j.bbrc.2015.04.103

実験手法

X-RAY DIFFRACTION (1.25 Å)