3X1Z
Ras-related protein Rap1B(T65A) with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.420, 59.040, 68.070 |
| Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
| Resolution | 33.960 - 1.250 |
| R-factor | 0.1469 |
| Rwork | 0.145 |
| R-free | 0.17220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.960 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.051 | 0.433 |
| Number of reflections | 78079 | |
| <I/σ(I)> | 12.5 | 3 |
| Completeness [%] | 95.5 | 92.7 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M Lithium sulfate, 0.1M bis-tris pH5.5, 25%(w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
