3WUM

Crystal structure of hen egg-white lysozyme

Summary for 3WUM

• Entry DOI: 10.2210/pdb3wum/pdb
• Related: 3WUL 3WUM
• Descriptor: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: serial femtosecond crystallography, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14389.60

Authors

Sugahara, M.,Nango, E.,Suzuki, M. (deposition date: 2014-04-28, release date: 2014-11-05, Last modification date: 2024-10-16)

Primary citation

Sugahara, M.,Mizohata, E.,Nango, E.,Suzuki, M.,Tanaka, T.,Masuda, T.,Tanaka, R.,Shimamura, T.,Tanaka, Y.,Suno, C.,Ihara, K.,Pan, D.,Kakinouchi, K.,Sugiyama, S.,Murata, M.,Inoue, T.,Tono, K.,Song, C.,Park, J.,Kameshima, T.,Hatsui, T.,Joti, Y.,Yabashi, M.,Iwata, S.
Grease matrix as a versatile carrier of proteins for serial crystallography
Nat.Methods, 12:61-63, 2015

PubMed Abstract: Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

PubMed: 25384243
DOI: 10.1038/nmeth.3172

Experimental method

X-RAY DIFFRACTION (2 Å)