3WTP

Crystal Structure of the heterotypic nucleosome containing human CENP-A and H3.3

3WTP の概要

• エントリーDOI: 10.2210/pdb3wtp/pdb
• 分子名称: Histone H3-like centromeric protein A, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: histone fold, dna binding, chromatin formation, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P49450 P62805 P04908 P06899 P84243
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202808.05

構造登録者

Arimura, Y.,Shirayama, K.,Horikoshi, N.,Fujita, R.,Kagawa, W.,Fukagawa, T.,Almouzni, G.,Kurumizaka, H. (登録日: 2014-04-14, 公開日: 2014-12-03, 最終更新日: 2023-11-08)

主引用文献

Arimura, Y.,Shirayama, K.,Horikoshi, N.,Fujita, R.,Taguchi, H.,Kagawa, W.,Fukagawa, T.,Almouzni, G.,Kurumizaka, H.
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
Sci Rep, 4:7115-7115, 2014

PubMed Abstract: The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle and reveal its "hybrid structure", in which the physical characteristics of CENP-A and H3.3 are conserved independently within the same particle. The CENP-A/H3.3 nucleosome forms an unexpectedly stable structure as compared to the CENP-A nucleosome, and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing cells.

PubMed: 25408271
DOI: 10.1038/srep07115

実験手法

X-RAY DIFFRACTION (2.67 Å)