3WTK

Crystal Structure of Lymnaea stagnalis Acetylcholine-Binding Protein Q55R Mutant Complexed with Thiacloprid

Summary for 3WTK

• Entry DOI: 10.2210/pdb3wtk/pdb
• Related: 2ZJU 2ZJV 3wth 3wti 3wtj 3wtl 3wtm 3wtn 3wto
• Descriptor: Acetylcholine-binding protein, {(2Z)-3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene}cyanamide (3 entities in total)
• Functional Keywords: neonicotinoids, nicotinic acetylcholine receptor, thiacloprid, acetylcholine binding, signaling protein
• Biological source: Lymnaea stagnalis (Great pond snail)
• Cellular location: Secreted: P58154
• Total number of polymer chains: 5
• Total formula weight: 122517.99

Authors

Okajima, T.,Ihara, M.,Yamashita, A.,Oda, T.,Matsuda, K. (deposition date: 2014-04-11, release date: 2015-02-04, Last modification date: 2024-11-06)

Primary citation

Ihara, M.,Okajima, T.,Yamashita, A.,Oda, T.,Asano, T.,Matsui, M.,Sattelle, D.B.,Matsuda, K.
Studies on an acetylcholine binding protein identify a basic residue in loop G on the beta 1 strand as a new structural determinant of neonicotinoid actions
Mol.Pharmacol., 86:736-746, 2014

PubMed Abstract: Neonicotinoid insecticides target insect nicotinic acetylcholine receptors (nAChRs). Their widespread use and possible risks to pollinators make it extremely urgent to understand the mechanisms underlying their actions on insect nAChRs. We therefore elucidated X-ray crystal structures of the Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP) and its Gln55Arg mutant, more closely resembling insect nAChRs, in complex with a nitromethylene imidacloprid analog (CH-IMI) and desnitro-imidacloprid metabolite (DN-IMI) as well as commercial neonicotinoids, imidacloprid, clothianidin, and thiacloprid. Unlike imidacloprid, clothianidin, and CH-IMI, thiacloprid did not stack with Tyr185 in the wild-type Ls-AChBP, but did in the Gln55Arg mutant, interacting electrostatically with Arg55. In contrast, DN-IMI lacking the NO2 group was directed away from Lys34 and Arg55 to form hydrogen bonds with Tyr89 in loop A and the main chain carbonyl of Trp143 in loop B. Unexpectedly, we found that several neonicotinoids interacted with Lys34 in loop G on the β1 strand in the crystal structure of the Gln55Arg mutant. Basic residues introduced into the α7 nAChR at positions equivalent to AChBP Lys34 and Arg55 enhanced agonist actions of neonicotinoids, while reducing the actions of acetylcholine, (-)-nicotine, and DN-IMI. Thus, not only the basic residues in loop D, but also those in loop G determine the actions of neonicotinoids. These novel findings provide new insights into the modes of action of neonicotinoids and emerging derivatives.

PubMed: 25267717
DOI: 10.1124/mol.114.094698

Experimental method

X-RAY DIFFRACTION (2.69 Å)