3WI3

Crystal Structure of the Sld3/Treslin domain from yeast Sld3

3WI3 の概要

• エントリーDOI: 10.2210/pdb3wi3/pdb
• 分子名称: DNA replication regulator SLD3, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: replication regulator, cdc45-binding, alpha helical
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Nucleus (By similarity): P53135
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 104509.47

構造登録者

Itou, H.,Araki, H.,Shirakihara, Y. (登録日: 2013-09-05, 公開日: 2014-08-20, 最終更新日: 2024-03-20)

主引用文献

Itou, H.,Muramatsu, S.,Shirakihara, Y.,Araki, H.
Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin.
Structure, 22:1341-1347, 2014

PubMed Abstract: The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed.

PubMed: 25126958
DOI: 10.1016/j.str.2014.07.001

実験手法

X-RAY DIFFRACTION (2.4 Å)