3WG7

A 1.9 angstrom radiation damage free X-ray structure of large (420KDa) protein by femtosecond crystallography

Summary for 3WG7

DescriptorCytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 2, Cytochrome c oxidase subunit 3, ... (28 entities in total)
Functional Keywordselectron transport complex iv, iron, oxidation-reduction, protein interaction domains, motifs, oxidoreductase, membrane
Biological sourceBos taurus (bovine,cow,domestic cattle,domestic cow)
Cellular locationMitochondrion inner membrane; Multi-pass membrane protein P00396 P68530 P00415
Mitochondrion inner membrane P00423 P00426 P00428 P07471 P04038 P07470 P13183 P00430 P10175
Mitochondrion intermembrane space P00429
Total number of polymer chains26
Total molecular weight441900.85
Authors
Primary citation
Hirata, K.,Shinzawa-Itoh, K.,Yano, N.,Takemura, S.,Kato, K.,Hatanaka, M.,Muramoto, K.,Kawahara, T.,Tsukihara, T.,Yamashita, E.,Tono, K.,Ueno, G.,Hikima, T.,Murakami, H.,Inubushi, Y.,Yabashi, M.,Ishikawa, T.,Yamamoto, M.,Ogura, T.,Sugimoto, H.,Shen, J.R.,Yoshikawa, S.,Ago, H.
Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
Nat.Methods, 2014
PubMed: 24813624 (PDB entries with the same primary citation)
DOI: 10.1038/nmeth.2962
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.9 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.248110.7%3.2%10.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3wg7
no rotation
Molmil generated image of 3wg7
rotated about x axis by 90°
Molmil generated image of 3wg7
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 3wg7
no rotation
Molmil generated image of 3wg7
rotated about x axis by 90°
Molmil generated image of 3wg7
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3wg7.pdb2.gz [341.23 KB])
Coordinate files for Biological unit (3wg7.pdb1.gz [345.86 KB])