3WEF

Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate

3WEF の概要

• エントリーDOI: 10.2210/pdb3wef/pdb
• 関連するPDBエントリー: 3VJ8 3VJ9 3VJA 3VJB 3VJC 3VJD 3WEG 3WEH 3WEI 3WEJ 3WEK
• 分子名称: Squalene synthase, S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL] TRIHYDROGEN THIODIPHOSPHATE (3 entities in total)
• 機能のキーワード: farnesyl-diphosphate farnesyltransferase, head-to-head synthases, cholesterol biosynthesis, oxidoreductase, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 239880.95

構造登録者

Liu, C.I.,Jeng, W.Y.,Wang, A.H.J. (登録日: 2013-07-07, 公開日: 2014-02-12, 最終更新日: 2023-11-08)

主引用文献

Liu, C.I.,Jeng, W.Y.,Chang, W.J.,Shih, M.F.,Ko, T.P.,Wang, A.H.J.
Structural insights into the catalytic mechanism of human squalene synthase.
Acta Crystallogr.,Sect.D, 70:231-241, 2014

PubMed Abstract: Squalene synthase (SQS) is a divalent metal-ion-dependent enzyme that catalyzes the two-step reductive `head-to-head' condensation of two molecules of farnesyl pyrophosphate to form squalene using presqualene diphosphate (PSPP) as an intermediate. In this paper, the structures of human SQS and its mutants in complex with several substrate analogues and intermediates coordinated with Mg2+ or Mn2+ are presented, which stepwise delineate the biosynthetic pathway. Extensive study of the SQS active site has identified several critical residues that are involved in binding reduced nicotinamide dinucleotide phosphate (NADPH). Based on mutagenesis data and a locally closed (JK loop-in) structure observed in the hSQS-(F288L)-PSPP complex, an NADPH-binding model is proposed for SQS. The results identified four major steps (substrate binding, condensation, intermediate formation and translocation) of the ordered sequential mechanisms involved in the `1'-1' isoprenoid biosynthetic pathway. These new findings clarify previous hypotheses based on site-directed mutagenesis and biochemical analysis.

PubMed: 24531458
DOI: 10.1107/S1399004713026230

実験手法

X-RAY DIFFRACTION (2.35 Å)