3WBZ

Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with ATP

3WBZ の概要

• エントリーDOI: 10.2210/pdb3wbz/pdb
• 関連するPDBエントリー: 3WC0 3WC1 3WC2
• 分子名称: Likely histidyl tRNA-specific guanylyltransferase, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Candida albicans (Yeast)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 270212.20

構造登録者

Nakamura, A.,Nemoto, T.,Sonoda, T.,Yamashita, K.,Tanaka, I.,Yao, M. (登録日: 2013-05-24, 公開日: 2013-12-18, 最終更新日: 2023-11-08)

主引用文献

Nakamura, A.,Nemoto, T.,Heinemann, I.U.,Yamashita, K.,Sonoda, T.,Komoda, K.,Tanaka, I.,Soll, D.,Yao, M.
Structural basis of reverse nucleotide polymerization
Proc.Natl.Acad.Sci.USA, 110:20970-20975, 2013

PubMed Abstract: Nucleotide polymerization proceeds in the forward (5'-3') direction. This tenet of the central dogma of molecular biology is found in diverse processes including transcription, reverse transcription, DNA replication, and even in lagging strand synthesis where reverse polymerization (3'-5') would present a "simpler" solution. Interestingly, reverse (3'-5') nucleotide addition is catalyzed by the tRNA maturation enzyme tRNA(His) guanylyltransferase, a structural homolog of canonical forward polymerases. We present a Candida albicans tRNA(His) guanylyltransferase-tRNA(His) complex structure that reveals the structural basis of reverse polymerization. The directionality of nucleotide polymerization is determined by the orientation of approach of the nucleotide substrate. The tRNA substrate enters the enzyme's active site from the opposite direction (180° flip) compared with similar nucleotide substrates of canonical 5'-3' polymerases, and the finger domains are on opposing sides of the core palm domain. Structural, biochemical, and phylogenetic data indicate that reverse polymerization appeared early in evolution and resembles a mirror image of the forward process.

PubMed: 24324136
DOI: 10.1073/pnas.1321312111

実験手法

X-RAY DIFFRACTION (2.392 Å)